MDRS Crew 25 has defined three major objectives for the mission: conducting directed research in the field and laboratory, integrating the Mars Rover into simulation, and making engineering improvements in the Hab.

The directed research involves various geological, biological and human factor studies conducted under the direction of the Remote Science Team (RST). The RST specifies the experimental procedures and EVA locations for the crew, who will perform the research and discuss the results with the RST. This telescience approach to field research is intended to investigate the feasibility of conducting research not planned by the actual crew, and without requiring the presence of a team of scientists specializing in those fields.

Integration of the Mars Rover into simulation involves a similar approach, in working via email with members of the PSU Mars Society (PSUMS) not participating in the simulation. The tele-engineering approach will allow the crew to complete repairs and updates on Rover design without needing an entire team of people. With the help of the support team, working on the Rover during simulation with limited resources will be more easily accomplished. Later, the Rover will be taken on EVAs and used for taking pictures and collecting samples in order to determine the extent to which a Rover can increase efficiency in the field.

In the Hab, many engineering improvements, small and large, can be implemented to make life in the Hab safer, more comfortable, and more realistic for MDRS participants. Projects currently planned include an elevation stand for the diesel generator, adding an inside door handle to the front airlock, and upgrades to the GreenHab power system.